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pact pe2 hygror  (Addgene inc)


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    Addgene inc pact pe2 hygror
    Pact Pe2 Hygror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pact pe2 hygror/product/Addgene inc
    Average 92 stars, based on 5 article reviews
    pact pe2 hygror - by Bioz Stars, 2026-03
    92/100 stars

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    Prime editing in cultured S2R+ cells. (A) Diagram of <t>PE2</t> expression plasmid <t>pUAS-PE2.</t> <t>attB,</t> phiC31 recombination site; NLS, nuclear localization sequence; PBS, primer-binding site; SV40, 3′ untranslated region; UAS, upstream activating sequence; w+, white+ rescue transgene. (B) Diagram of pCFD3-NS pegRNA expression plasmid. BbsI sites indicate cloning site for pegRNA encoding sequence. dU6:3, U6 promoter; U6 3′, U6 downstream region; v+, vermillion+ rescue transgene. (C) ebony genomic region showing target site and edit (ebonyG111X). (D) Dual sgRNA and pegRNA expression plasmid pCFD5-NS. tRNA, D. melanogaster and O.s. Gly tRNA sequence. (E) Schematic of S2R+ prime editing experiment. (F) Approximate quantification of precise editing and indels from S2R+ transfection experiments by amplicon sequencing. tfx, transfection.
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    pact-pe2-hygror  (Addgene inc)
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    Prime editing in cultured S2R+ cells. (A) Diagram of <t>PE2</t> expression plasmid <t>pUAS-PE2.</t> <t>attB,</t> phiC31 recombination site; NLS, nuclear localization sequence; PBS, primer-binding site; SV40, 3′ untranslated region; UAS, upstream activating sequence; w+, white+ rescue transgene. (B) Diagram of pCFD3-NS pegRNA expression plasmid. BbsI sites indicate cloning site for pegRNA encoding sequence. dU6:3, U6 promoter; U6 3′, U6 downstream region; v+, vermillion+ rescue transgene. (C) ebony genomic region showing target site and edit (ebonyG111X). (D) Dual sgRNA and pegRNA expression plasmid pCFD5-NS. tRNA, D. melanogaster and O.s. Gly tRNA sequence. (E) Schematic of S2R+ prime editing experiment. (F) Approximate quantification of precise editing and indels from S2R+ transfection experiments by amplicon sequencing. tfx, transfection.
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    Prime editing in cultured S2R+ cells. (A) Diagram of PE2 expression plasmid pUAS-PE2. attB, phiC31 recombination site; NLS, nuclear localization sequence; PBS, primer-binding site; SV40, 3′ untranslated region; UAS, upstream activating sequence; w+, white+ rescue transgene. (B) Diagram of pCFD3-NS pegRNA expression plasmid. BbsI sites indicate cloning site for pegRNA encoding sequence. dU6:3, U6 promoter; U6 3′, U6 downstream region; v+, vermillion+ rescue transgene. (C) ebony genomic region showing target site and edit (ebonyG111X). (D) Dual sgRNA and pegRNA expression plasmid pCFD5-NS. tRNA, D. melanogaster and O.s. Gly tRNA sequence. (E) Schematic of S2R+ prime editing experiment. (F) Approximate quantification of precise editing and indels from S2R+ transfection experiments by amplicon sequencing. tfx, transfection.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Precise genome engineering in Drosophila using prime editing

    doi: 10.1073/pnas.2021996118

    Figure Lengend Snippet: Prime editing in cultured S2R+ cells. (A) Diagram of PE2 expression plasmid pUAS-PE2. attB, phiC31 recombination site; NLS, nuclear localization sequence; PBS, primer-binding site; SV40, 3′ untranslated region; UAS, upstream activating sequence; w+, white+ rescue transgene. (B) Diagram of pCFD3-NS pegRNA expression plasmid. BbsI sites indicate cloning site for pegRNA encoding sequence. dU6:3, U6 promoter; U6 3′, U6 downstream region; v+, vermillion+ rescue transgene. (C) ebony genomic region showing target site and edit (ebonyG111X). (D) Dual sgRNA and pegRNA expression plasmid pCFD5-NS. tRNA, D. melanogaster and O.s. Gly tRNA sequence. (E) Schematic of S2R+ prime editing experiment. (F) Approximate quantification of precise editing and indels from S2R+ transfection experiments by amplicon sequencing. tfx, transfection.

    Article Snippet: The Act5c fragment was inserted into the pMK33-GW backbone by Gibson assembly. pUAS-PE2-attB (Addgene 149550; DGRC 1527) and pAct-PE2-HygroR (Addgene; 149552) were generated by Gateway reactions between pEntr_PE2 and pWalium10-roe ( 57 ) or pAct-GW-HygroR , respectively.

    Techniques: Cell Culture, Expressing, Plasmid Preparation, Sequencing, Binding Assay, Clone Assay, Transfection, Amplification